The resulting serial dilution series has a dilution factor of approximately 1. Remember, keep going until youre very comfortable with the calculations. Sample math problems for microbiology david fankhauser. Dilution techniques and pipetting biology libretexts. There are three formulas used to work microbiology dilution problems.
Molarity practice problems this chemistry video tutorial explains how to solve common molarity problems. Concentrations and dilutions answer key displaying top 8 worksheets found for this concept some of the worksheets for this concept are dilutions work, dilutions work, dilutions work name key, dilutions work w 329, concentrations and dilutions, molarity and serial dilutions teacher handout, laboratory math ii solutions and dilutions. Well, that formula, c i v i c f v f, is a perfectly good one to use for most types of dilution problems, as long as the units on both sides of the equation match. If it is, the sample dilution factor will be shown in the diagram as above the 12 in the erlenmeyer flask is the sample dilution factor. Once you find your worksheet, click on popout icon or print icon to worksheet to print or download. The exercise will allow students to learn how to pipette, dilute samples and calculate the concentration of their samples. Lab math solutions, dilutions, concentrations and molarity.
The most common type of dilutions done in chemistry lab involve taking a sample of a solution of known volume and concentration and diluting it to some larger volume. If students are not familiar with operating pipettes, the pre lab serial dilutions practice exercise is recommended. It is a common practice to determine bacterial counts for both liquid and solid specimenssuspensions of e. Dilution is the addition of solvent, which decreases the. A dilution is the mixing of a small accurately measured sample with a large volume of diluent usually sterile water or saline solution 1. Some of the worksheets displayed are dilutions work, chemistry dilution practice, dilutions work w 329, dilutions work, making dilutions work, molarity molarity, chem1001 work 6 concentration model 1 concentration, concentration work w 328.
Dilution factor practice problem to dilute a bacterial culture, 500. So you multiply each successive dilution by the dilution factor. Here you will find all we have for dilution worksheet. This worksheet and quiz will let you practice the following skills. Key is licensed under a creative commons attributionnoncommercialsharealike 4. Concentrations and dilutions answer key kiddy math. Answers serial dilutions practice worksheet biol 307 studocu. Dilution and concentration theory dilution and concentration theory sounds ominous but you use dilutions and concentrations fairly frequently outside of your scientific life.
Displaying all worksheets related to dilution problems. Reading comprehension ensure that you draw the most. Answers serial dilutions practice worksheet biol 307. The answers i sure hope you tried to answer the problems before you looked here. Dilutions worksheet solutions 1 if i add 25 ml of water to 125 ml of a 0. Defining key concepts ensure that you can accurately define main phrases, such as solution and molarity. This type of dilution series is referred to as a serial dilution. In fact, it relies on the principle used in the above calculations. Serial dilution many procedures performed in modern biology and chemistry laboratories require sets of solutions that cover a range of concentration s. In the lab, correct dilutions of solutions are critical to performing accurate science. Serial dilutions practice problems refer to the below image. Easy speed math they dont teach you in school part 1 addition duration.
Some of the worksheets displayed are chemistry dilution practice, dilutions work, dilutions work, dilutions work w 329, dilution work answers, extra molarity problems for practice, working dilution problems, molarity and serial dilutions teacher handout. Often, a worker will need to change the concentration of a solution by changing the amount of solvent. Determine the concentration or dilution factor for the flasks and plates. A set of serial dilutions is made, a sample of each is placed into a liquefied agar medium, and the medium poured into a petri dish. Here you will find all we have for dilution problems worksheet. Learn vocabulary, terms, and more with flashcards, games, and other study tools.
All materials were written for and are currently used. Dilutions worksheet w 329 everett community college. This worksheet defines stock solution, serial dilution and provides 3 practice problems. We worked through the first example together on the board and the students did the dilution using coloured water. Serial dilution protocol pdf it is a method of diluting a stock solution where concentration decreases by the same quantity in each successive step. Dilutions are useful in science when making solutions or growing an acceptable number of bacterial colonies to count. Determine how many moles of solute and liters of solvent you have for each solution you are. In this dilutions worksheet, students calculate molarity of solutions and practice figuring out the volume of concentrate needed for a specific dilution of solutions. Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume.
Calculating final dilution in a given serial dilution problem skills practiced. Pltw, mi unit 1 serial dilution practice quiz quizizz. Dilution displaying top 8 worksheets found for this concept some of the worksheets for this concept are dilutions work, dilutions work w 329, dilution name chem work 15 5, dilutions work, dilution work answers, chemistry dilution practice, dilutions work name key, solutions work 2 molarity and dilution problems answers. These include quantifying the number of bacteria in a sample using plate counts and the development of standard curves for quantitative colorimetric, radiometric, and enzymatic assays. In the 1st of your small containers, place 10 drops of food coloring. In each of the rest of your small containers, place 9 drops of water. Color the first circle the color of the container with food coloring or describe the color in the circle. Showing top 8 worksheets in the category dilution problems. The following problem sets test your ability to calculate dilution factors and concentrationsdilution factor calculationconcentration of a dilution. This is just one of the solutions for you to be successful. Working dilution problems it is a common practice to determine microbial counts for both liquid and solid specimenssuspensions of e.
To determine the concentration you multiply the number of colonies by the inverse of the dilution factor in flask b it was 104. Serial dilution displaying top 8 worksheets found for this concept some of the worksheets for this concept are dilutions work, dilutions work w 329, molarity and serial dilutions teacher handout, dilutions work, laboratory math ii solutions and dilutions, lab math solutions dilutions concentrations and molarity, well what will we drink, dilutions work name key. A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step in serial dilutions, you multiply the dilution factors for each step. In medicine, these skills are necessary to ensuring proper medicine dosage and treatment. Sample dilution factor sdf a sample is often diluted prior to doing the serial dilutions. The following is a stepbystep procedure to working dilution problems, and includes some practice problems at the end. Aug, 1995 a dilution of a bacterial suspension was prepared by adding 100 lambdas of the suspension to 9. To make a dilution series, use the following formulas.
Below is an applet to practice finding dilution factors, and also to determine how much water to add to achieve a given dilution. Dilution is a decrease in a solutions concentration, whereas concentration is an increase in a solutions concentration. A serial dilution is a series of simple dilutions which amplifies the dilution factor quickly. A dilution of a bacterial suspension was prepared by adding 100 lambdas of the suspension to 9. Molarity and serial dilutions teacher handout module overview one of the most important skills in science is to calculate dilutions. This is a chemistry tutorial that covers dilution problems, including examples of how to calculate the new concentration of a diluted solution, and how to calculate the volume of a concentrated. Worksheets are dilutions work, dilutions work w 329, molarity and serial dilutions teacher handout, dilutions work name key, laboratory math ii solutions and dilutions, making dilutions work, lab math solutions dilutions concentrations and molarity, lab basic skills dilutions micropipettes and. How to work microbiology dilution problems sciencing. Dilution of solutions worksheet 1 free download dilution of solutions worksheet. In a serial dilution, the final total dilution is a product of each individual dilution in the series. If one ml was spread on plate a it would be x 10 4 or 000 or 1. Worksheets are chemistry dilution practice, dilutions work, dilutions work, dilutions work w 329, dilution work answers, extra molarity problems for practice, working dilution problems, molarity and serial dilutions teacher handout. Dilutions worksheet w 329 everett community college student support services program 1 if 45 ml of water are added to 250 ml of a 0. Most specimens have high enough numbers of microorganisms that the specimen has to be serially diluted to quantitate effectively.
Dilutions and concentrations introductory chemistry. Thus, a series of 5, one to ten dilutions equals a one to one hundred thousand dilution. The purpose can be determination of bacterial, fungal, or viral counts indirectly. Dilutions worksheet 1 if i add 25 ml of water to 125 ml of a 0. Free gamified quizzes on every subject that students play in class and at home. I made this for my as biology students who dont take chemistry and needed some practice with serial dilutions before jumping straight into a full practical. Serial dilution practice problem set science primer. If the sample remains undiluted, use 11 as the sample dilution factor. Calculating final dilution in a given serial dilution problem. This worksheet and quiz let you practice the following skills. Nov 26, 2011 this is a chemistry tutorial that covers dilution problems, including examples of how to calculate the new concentration of a diluted solution, and how to calculate the volume of a concentrated. Dilutions worksheet socorro independent school district.
Good practice for those who have never used a syringe too. Once again, heres an applet to practice finding the total dilution scheme, regardless of how the dilution scheme is expressed as directions, as fractions, or as decimals. You will make several dilutions of a bacterial stock culture. The important thing to remember is that the amount of solute doesnt change during the dilution process, only the amount of solvent and. Click on popout icon or print icon to worksheet to. Dilutions worksheet awesome science teacher resources. Then 1 ml from flask a is transferred to flask b and then 1 ml from flask b is transferred to flask c. Jun 01, 20 the resulting serial dilution series has a dilution factor of approximately 1. Dilutions and concentrations introductory chemistry 1st. For example, when you are making orange juice from a frozen concentrate, you mix 1 can of concentrate with 3 cans of water. Reading comprehension ensure that you draw the most important information from the properties of serial dilution lesson. A dilution series is a succession of step dilutions, each with the same dilution factor, where the diluted material of the previous step is used to make the subsequent dilution. Most specimens have high enough numbers of microorganisms that. The following protocol is a stepbystep procedure to working dilution problems, and includes some practice problems at the end.
From the original culture 1 ml of broth was transferred to flask a which contains 99 ml of sterile water. Dilution problems worksheet worksheet fun and printable. Serial dilutions practice problems san diego miramar college. Displaying all worksheets related to serial dilutions.